Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media

نویسندگان

  • Birgit Weyand
  • Meir Israelowitz
  • James Kramer
  • Christian Bodmer
  • Mariel Noehre
  • Sarah Strauss
  • Elmar Schmälzlin
  • Christoph Gille
  • Herbert P. von Schroeder
  • Kerstin Reimers
  • Peter M. Vogt
چکیده

A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational fluid simulation can support design of bioreactor systems for tissue engineering application.

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عنوان ژورنال:

دوره 2015  شماره 

صفحات  -

تاریخ انتشار 2015